Tabriz University of Medical Sciences Journal of Research in Clinical Medicine 2717-0616 10 1 2022 01 01 Association of two high-risk strains of human papillomavirus (HPV18 and HPV16) with breast cancer in the patients using polymerase chain reaction 12 12 10.34172/jrcm.2022.012 EN Loghman Ghaderi https://orcid.org/0000-0002-0823-830X Majid Montazer https://orcid.org/0000-0001-7846-0128 Ali Reza Naseri https://orcid.org/0000-0001-9714-2379 Journal Article 10.34172/jrcm.2022.012 2021 02 16 2021 04 17 Introduction: Two high-risk strains of human papillomavirus (HPV18 and HPV16) immortalize human mammary epithelial cells and reduce their growth factor requirements. Recent studies have identified HPV in a broad range of breast cancer samples. Therefore, the study aimed to assess the association of the mentioned high-risk HPV strains (HPV18, HPV16) with breast cancer in the patients using polymerase chain reaction (PCR) due to the increasing prevalence of breast cancer. Methods: The current work was a retrospective study involving 60 participants (40 patients with breast cancer and 20 healthy people) at Tabriz University of Medical Sciences in 2018-2019. The participants were selected using a convenience sampling method. PCR was used to detect DNA genomes of HPV18 and HPV16. Data was analyzed using Phi and Cramér’s V with SPSS v. 21 by taking into account OR=95% and P=0.05. Results: There was a significant relationship (P value=0.014) between HPV18 and HPV16 infection and breast cancer. Conclusion: A significant relationship was found between the presence of DNA genomes of HPV16 and HPV18 and breast cancer. Therefore, HPV has a significant role in breast cancer.