Tabriz University of Medical Sciences Journal of Research in Clinical Medicine 2717-0616 2 4 2014 11 30 Early detection of Toxoplasma gondii by real-time polymerase chain reaction methods in patients with recurrent spontaneous abortions 193 196 10.5681/jarcm.2014.032 EN Parviz Saleh Mehrdad Asghari-Estiar Zoleikha Asgarlou Behjat Shokrvash Fatemeh Abbasalizadeh Ebrahim Sakhinia Fatemeh Mallah Reza Piri Mohammad Naghavi-Behzad Journal Article 10.5681/jarcm.2014.032 2014 07 12 Introduction: One of the causes of recurrent spontaneous abortions (RSA) is an infection by the toxoplasmosis Protozoa. In comparison, we present detailed results using real-time polymerase chain reaction (PCR) methods of detection. In this study, it was tried to detect Toxoplasma gondii (T. gondii) by real-time PCR methods in patients with RSA. Methods: Amniotic fluid sampling was performed in the 16-20th weeks of gestation in 50 pregnant women with a history of RSA. The extracted deoxyribonucleic acid (DNA) samples were analyzed using quantitative real-time PCR. Results: In all the cases, the detection of T. gondii was negative in the peripheral blood, and amniotic fluid samples by using the molecular methods (real-time PCR). Using the serological detection methods, 6% of patients were diagnosed as positive for the immunoglobulin M (IgM) antibody. In addition, the IgG antibody was positive in 46% of the patients. Conclusion: It can be concluded that the serological methods lack specificity.